Journal: Mammalian Genome

Article Title: The use of genistein and ambroxol may be an effective approach in correcting cellular dysfunctions of mucopolysaccharidosis-plus syndrome

doi: 10.1007/s00335-025-10176-2

Figure Lengend Snippet: Abundance of the Rab7 protein in control (healthy) and MPSPS fibroblasts either untreated or treated with 0.1% DMSO (solvent), 50 µM genistein (G50), 100 µM ambroxol (AMB100) or a mixture of these two compounds (G50 + AMB100) as estimated by Western-blotting (representative results are shown in upper panel; total protein module was used as a loading control), with quantification performed using densitometry (lower panel). The quantitative results are presented as mean values from 3 independent experiments, with error bars indicating SD. The mean value obtained for control (healthy) cells was considered as 1, and other values reflect this value. Statistically significant differences ( p < 0.05) are indicated by asterisks (*) - relative to control (healthy) cells; hashtags (#) - to untreated or DMSO-treated MPSPS cells (both comparisons gave the same assessment of significance of the differences), dollar symbol ($) - to MPSPS cells treated with genistein; and ampersand (&) - to MPSPS cells treated with ambroxol

Article Snippet: Proteins (including Rab5, Rab7, VPS33A, and VPS16) were separated using the 12–230 kDa separation module (#SM-W003) and detected in-capillary using specific primary antibodies: anti-Rab5 (#3547, Cell Signaling), anti-Rab7 (D95F2, #9367, Cell Signaling), anti-VPS33A (#16896-1-AP, ProteinTech), and anti-VPS16 (#17776-1-AP, ProteinTech).

Techniques: Control, Solvent, Western Blot

Journal: Mammalian Genome

Article Title: The use of genistein and ambroxol may be an effective approach in correcting cellular dysfunctions of mucopolysaccharidosis-plus syndrome

doi: 10.1007/s00335-025-10176-2

Figure Lengend Snippet: Abundance of the Rab7 protein in control (healthy) and MPSPS fibroblasts either untreated or treated with 0.1% DMSO (solvent), 50 µM genistein (G50), 100 µM ambroxol (AMB100) or a mixture of these two compounds (G50 + AMB100) as estimated by fluorescence microscopy using Rab7-specific antibody (representative results are shown in upper panel; bars represent 10 μm), with quantification performed using a software provided by the microscope manufacturer (lower panel). At least 100 cells were analyzed in each experimental variant. The quantitative results are presented as mean values from 3 independent experiments, with error bars indicating SD. The mean value obtained for control (healthy) cells was considered as 1, and other values reflect this value. Statistically significant differences ( p < 0.05) are indicated by asterisks (*) - relative to control (healthy) cells; hashtags (#) - to untreated or DMSO-treated MPSPS cells (both comparisons gave the same assessment of significance of the differences), dollar symbol ($) - to MPSPS cells treated with genistein; and ampersand (&) - to MPSPS cells treated with ambroxol

Article Snippet: Proteins (including Rab5, Rab7, VPS33A, and VPS16) were separated using the 12–230 kDa separation module (#SM-W003) and detected in-capillary using specific primary antibodies: anti-Rab5 (#3547, Cell Signaling), anti-Rab7 (D95F2, #9367, Cell Signaling), anti-VPS33A (#16896-1-AP, ProteinTech), and anti-VPS16 (#17776-1-AP, ProteinTech).

Techniques: Control, Solvent, Fluorescence, Microscopy, Software, Variant Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: RAB23 facilitates clathrin-coated nascent vesicle formation at the plasma membrane and modulates cell signaling

doi: 10.1007/s00018-025-05694-w

Figure Lengend Snippet:

Article Snippet: Endosomal Marker Antibody Sampler Kit: Rabbit anti-EEA1 Rabbit anti-RAB5 Rabbit anti-RAB7 Rabbit anti-RAB11 Rabbit anti-Clathrin Rabbit anti-Caveolin 1 , Cell signaling technology , Cat#12666.

Techniques: Marker, Recombinant, Lysis, Blocking Assay, Protease Inhibitor, Transfection, Bicinchoninic Acid Protein Assay, Derivative Assay, Plasmid Preparation, Software, Imaging

Journal: PLoS ONE

Article Title: Tumor Imaging and Targeting Potential of an Hsp70-Derived 14-Mer Peptide

doi: 10.1371/journal.pone.0105344

Figure Lengend Snippet: MCF7 tumor cells grown in glass-bottomed MatTek dishes for 48 h were fixed in paraformaldehyde, permeabilized and stained with primary antibodies specific for early (Rab4, Rab5), late (Rab7, Rab9) and recycling (Rab11) endosomes or lysosomes (LAMP1), followed by an appropriate Cy3-labeled secondary antibody (red). The nucleus was counter-stained with DAPI (blue). Representative three-frame composites from across the z-stack are shown. All of the vesicular markers assessed were found to be homogenously distributed throughout the cells. Objective 63×; scale bar 10 µm.

Article Snippet: Monoclonal antibodies recognizing Rab4, Rab5, Rab7, Rab9 and Rab11 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.) and antibodies recognizing LAMP1 and LAMP2 were obtained from Sigma-Aldrich (St. Louis, MO, U.S.A.).

Techniques: Staining, Labeling